Angular Mapping of Protein Structure Using Nonlinear Optical Measurements

Author links open overlay panelClancy, B.; Moree, B.; Salafsky, J. Biophys. J (2019)

Second-harmonic generation-based methods to detect and characterize ligand-induced RNA conformational changes

Birman, Y.; Khorsand, S.; Tu, E.; Mortensen, R.B.; Butko, M.T.; Methods (2019).

Engineering a Single-Agent Cytokine/Antibody Fusion That Selectively Expands Regulatory T Cells for Autoimmune Disease Therapy

Spangler, J.B.; Trotta, E.; Tomala, J.; Peck, A.; Young, T.A.; Savvides, C.S.; Silveria, S.; Votavova, P.; Salafsky, J.; Pande, V.S.; Kovar, M.; Bluestone, J.A.; Garcia, K.C.; J Immunol. (2018).

Second-Harmonic Generation (SHG) for Conformational Measurements: Assay Development, Optimization, and Screening

Young, T.A.; Moreee, B.; Butko, M.T.; Clancy, B.; Do, M.; Gheyi, T.; Strelow, J.; Carrillo, J.J.; Salafsky, J.;  Methods Enzymol. (2018).

Identification of Inactive Conformation‐Selective Interleukin‐2‐inducible T‐cell Kinase (ITK) Inhibitors based on Second‐Harmonic Generation

Discovery of Selective RNA-Binding Small Molecules by Affinity-Selection Mass Spectrometry

Rizvi, N.F.; Howe, J.A.; Nahvi, A.; Klein, D.J.; Fischmann, T.O.; Kim, H.Y.; McCoy, M.A.; Walker, S.S.; Hruza, A.; Richards, MP.; Chamberlin, C.; Saradjian, P.; Butko, M.T.; Mercado, G.; Burchard, J.; Strickland, C.; Dandliker, PJ.; Smith, G.F.; Nickbarg, E.B.; ACS Chem Biol. (2018).

Monomeric ephrinB2 binding induces allosteric changes in Nipah virus G that precede its full activation

Wong, J.J.W.; Young, T.A.; Zhang, J.; Liu, S.; Leser, G.P.; Kornives, E.A; Lamb, R.A; Hong Zhou, Z.; Salafsky, J.; Jardetzky, T.S.; Nature Communications. (2017).

Evaluating the contribution of transition state destabilization to changes in the residence time of triazole-based InhA inhibitors

Spagnuolo, L.A.; Eltschkner, S.; Yu, W.; Daryaee, F.; Davoodi, S.; Knudson, S.E.; Allen, E.K.H.; Merino, J.; Pschibul, A.; Moree, B.; Thivalapill, N.; Truglio, J.J.; Salafsky, J.; Slayden, R.A.; Kisker, C. and Tonge, P.J.; Journal of the American Chemical Society. (2017).

Second-harmonic phase determination by real-time in situ interferometry

Clancy, B. and Salafsky, J. Physical Chemistry Chemical Physics. 19, 3722-3728 (2017).

ATP-Competitive MLKL Binders Have No Functional Impact on Necroptosis

Ma, B.; Marcotte, D.; Paramasivam, M.; Michelsen, K.; Wang, T.; Bertolotti-Ciarlet, A.; Jones, J.H.; Moree, B.; Butko, M.; Salafsky, J.; Sun, X.; McKee, T. and Silvian, L.F. PLoS ONE 11 e0165983 (2016).

Detection of ligand-induced conformational changes in oligonucleotides by second-harmonic generation at a supported lipid bilayer interface

Butko, M.T; Moree, B.; Mortensen, R.B. and Salafsky, J. Analytical Chemistry. 88 (21), pp 10482–10489 (2016).

Small Molecules Detected by Second-Harmonic Generation Modulate the Conformation of Monomeric Alpha-Synuclein and Reduce its Aggregation in Cells

Moree, B.; Yin G.; Lázaro, D.F.; Munari, F.; Strohäker, T.; Giller, K.; Becker, S.; Outeiro, T.F.; Zweckstetter, M. and Salafsky, J. The Journal of Biological Chemistry. 290, 27582 – 27593 (2015).

Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation

Moree, B.; Connell, K.; Mortensen, R.B.; Liu, C.T.; Benkovic, S.J. and Salafsky, J. Biophysical Journal. 109, 806 – 815 (2015)

A second-harmonic-active unnatural amino acid as a structural probe of biomolecules on surfaces

Salafsky, J.S and Cohen, B. A. The Journal of Physical Chemistry B. 112, 15103 – 15107 (2008).

Second-harmonic generation for studying structural motion of biological molecules in real time and space

Salafsky, J.S. Physical Chemistry Chemical Physics. 9, 5704 – 5711 (2007).

Detection of protein conformational change by optical second-harmonic generation

Salafsky, J.S. The Journal of Chemical Physics. 125, 074701 (2006).

Second-harmonic generation as a probe of conformational change in molecules

Salafsky, J.S. Chemical Physics Letters. 381, 705 – 709 (2003).

External Reviews that Reference Our Technology

Biophysics: for HTS hit validation, chemical lead optimization, and beyond

Genick, C.C.; Wright S.K.; Expert Opin Drug Discov. (2017).

Biophysics in drug discovery: impact, challenges and opportunities

Renaud, J.P.; Chung, C.; Danielson, U.H.; Egner, U.; Hennig, M.; Hubbard, R.E.; Nar, H.; Nat. Rev. Drug Discov. (2016).